Regeneration of mouse uterus using decellularized uterine matrix transplantation

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15700
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: The University of Tokyo
• Principal Investigator: Osuga Yutaka 東京大学, 医学部附属病院, 教授 (80260496)
• Co-Investigator(Kenkyū-buntansha): 廣田 泰 東京大学, 医学部附属病院, 講師 (40598653)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 子宮 / 脱細胞化 / 再生 / STAT3 / 着床 / 細胞外基質 / 妊娠

Outline of Final Research Achievements

The aim of this study is to establish a new therapeutic strategy for implantation failure. We established a method of uterine regeneration through decellularized uterine matrix transplantation in mice. The decellularized uterine matrices obtained after SDS treatment were transplanted into the recipient mouse uteri with artificially induced defects. All the uterine layers were recovered at the sites of decellularized uterine matrix transplantation within a month. In the regenerated uterus, normal implantation and pregnancy maintenance were observed. The tissue regeneration processes were totally impaired in mice with uterine deletion of STAT3, a downstream signaling pathway of LIF, suggesting that STAT3 has a critical role in uterine regeneration as well as implantation. Thus, decellularized uterine matrix is a possible tool for regeneration of the functional uterus.

Free Research Field

産婦人科学