In vivo functional genomics screen reveals mechanism of peritoneal dissemination in ovarian cancer

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15706
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Obstetrics and gynecology
• Research Institution: Kyoto University
• Principal Investigator: Konishi Ikuo 京都大学, 医学研究科, 名誉教授 (90192062)
• Co-Investigator(Kenkyū-buntansha): 松村 謙臣 近畿大学, 医学部, 教授 (20452336) ; 安彦 郁 京都大学, 医学(系)研究科(研究院), 助教 (20508246) ; 濱西 潤三 京都大学, 医学研究科, 講師 (80378736)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 卵巣癌

Outline of Final Research Achievements

A library of 81 000 shRNAs targeting 15 000 human genes was stably transfected to ovarian cancer cell lines. By functional screening of these cancer cells, we screened genes that are responsible for anoikis resistance when down-regulated. One of the screened genes was ABHD2, which was fuctionally related to cisplatin resistance. Furthermore, we identified genes that increase Side Population cells when down-regulated. Side Population cells are defined as cells which have ability to efflux Hoechst 33342. Down-regulation of these genes were related to cancer stemness phenotype.

Free Research Field

産婦人科