Bioengineering of transplantable small intestine using decellularized intestinal scaffold

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15742
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Pediatric surgery
• Research Institution: Kyoto University
• Principal Investigator: Okamoto Shinya 京都大学, 医学研究科, 客員研究員 (00329377)
• Co-Investigator(Kenkyū-buntansha): 安近 健太郎 京都大学, 医学研究科, 客員研究員 (00378895) ; 上本 伸二 京都大学, 医学研究科, 教授 (40252449) ; 石井 隆道 京都大学, 再生医科学研究所, 研究員 (70456789)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 小腸移植 / 小腸再生 / 脱細胞化小腸

Outline of Final Research Achievements

Although small bowel transplantation is the only curative treatment for patients with irreversible intestinal failure, graft failure rate remains high. Therefore, a new treatment strategy for intestinal failure is required. The aim of this study is to fabricate a transplantable and functional small intestine using 3-dimensional decellularized intestinal scaffold. After removal of the small intestine from Lewis rat, the decellularized small intestinal scaffold was prepared by perfusing detergent solution via the vessel. We implanted the decellularized small intestinal scaffold to rat, and histological analysis was performed. Infiltration of intestinal mucosal epithelium and smooth muscle cells in addition to angiogenesis into the decellularized intestinal scaffold could be observed. It was suggested that the decellularized small intestinal scaffold might promote regeneration of the small bowel with infiltration of small intestinal components from recipient.

Free Research Field

小児外科