Application of biomaterials for regulation of osteoblast differentiation to craniosynostosis

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15746
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Plastic surgery
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ISEKI Sachiko 東京医科歯科大学, 大学院医歯学総合研究科, 教授 (80251544)
• Co-Investigator(Kenkyū-buntansha): 由井 伸彦 東京医科歯科大学, 生体材料工学研究所, 教授 (70182665) ; 上田 晃一 大阪医科大学, 医学部, 教授 (90257858)
• Co-Investigator(Renkei-kenkyūsha): Arun Kumar Rajendran 東京医科歯科大学, 大学院医歯学総合研究科, 大学院生
• Research Collaborator: Arisaka YOSHINORI 東京医科歯科大学, 生体材料工学研究所, 助教
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: craniosynostosis / osteoblast / diffferentiation / polyrotaxane

Outline of Final Research Achievements

Craniosynostosis involves the premature bony closure of the cranial sutures, which is due to early and rapid ossification. The current treatment modality is surgical separation of the fused bones to create the space. However, since the osteoblasts are in very active state, repeated closure occurs, which requires repeated surgeries.
Polyrotaxane (PRX), one of biomaterials can regulate differentiation of mesenchymal stem cells by changing the mobility of PRX surface. We have intended to utilize this property to regulate osteoblast differentiation to development the treatment minimizing repeated surgeries.
We found that different molecular mobility of PRX affects proliferation, mineralization, cytoskeleton organization and YAP localization of different type of osteoblasts. We could utilize the molecular mobility of PRX to regulate osteogenesis for avoiding repeated surgeries.

Free Research Field

先天異常学、発生生物学、再生医療学