Preconditionong treatment for the rat model of acute subdural hematoma with stemcell transplantation

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K15768
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Emergency medicine
• Research Institution: Nippon Medical School
• Principal Investigator: Shoji Yokobori 日本医科大学, 医学部, 准教授 (70449271)
• Co-Investigator(Kenkyū-buntansha): 増野 智彦 日本医科大学, 医学部, 講師 (00318528) ; 須田 智 日本医科大学, 医学部, 講師 (00366733) ; 山田 真吏奈 日本医科大学, 医学部, 講師 (70508621)
• Research Collaborator: Bullock Ross ; Gajavelli Shyam
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 幹細胞移植 / 頭部外傷 / 急性硬膜下血腫 / 減圧開頭術

Outline of Final Research Achievements

The aim of this pilot study was to test feasibility using a rat ASDH decompression model with two clinically relevant transplantation methods. Two different methods, in situ stereotactic injection and hNSC-embedded matrix seating on the brain surface, were attempted. Athymic rats were randomized to uninjured or ASDH groups . Animals in injury group were subjected to ASDH, and received decompressive craniectomy and 1-week after decompression surgery were transplanted with green fluorescent protein (GFP)-transduced hNSCs using one of two approaches. Histopathological examinations at 4 and 8 weeks showed that the GFP-positive hNSCs survived in injured brain tissue. The in situ transplantation group had greater engraftment of hNSCs than matrix embedding approach. Motor function was assessed with rotarod, compared to control group (n = 10). The latency to fall from the rotarod in hNSC in situ transplanted rats was significantly higher than in control rats.

Free Research Field

脳神経外科　救急科

Academic Significance and Societal Importance of the Research Achievements

本研究により、神経外傷におけるヒト神経幹細胞の生着を確認できた。また、ステレオ下にIn situ　に移植するのみならず、減圧開頭術後の脳表面にシートにて移植することでも生着しうることが明らかになった。神経幹細胞をInsituに移植したほうが生着の割合は高かった。どちらの細胞も4-8週生着することが明らかになり、今後のわが国の脳神経外傷の治療に向けて前進するための見地が得られた。