2018 Fiscal Year Final Research Report
Analysis of process of tooth organogenesis using embryonic stem cells and attempt of tooth organogenesis using them
| Project/Area Number |
16K15774
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Mie University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
山根 利之 三重大学, 医学系研究科, 准教授 (30452220)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | 神経堤細胞 / 象牙芽細胞 / エナメル芽細胞 / 胚性幹細胞 / 歯の器官形成 / 蛍光標識 |
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| Outline of Final Research Achievements |
By using mouse embryonic stem cells, we found that cells from ES cell culture expressed Dspp or Amelx. To investigate if Dspp-expressing odontoblasts or Amelx-expressing ameloblasts were induced from ESCs in this culture, we had established Amelx-TdTomato Knock In (Amelx TdTomato/+) mice and Dspp-GFP knock In (Dspp GFP/+) mice to identify ameloblasts and odontoblasts as TdTomato-expressing cells, and GFP-expressing cells, respectively. Using these mice, we found that TdTomato and GFP were strongly expressed on ameloblasts and odontoblasts, respectively. Furthermore, we had established ES cell lines from these transgenic mice. Although we tried to induce GFP-expressing odontoblast and TdTomato-expressing ameloblasts from ESC cultures, we had not identified odontoblasts and ameloblasts from ESCs. Using cells from embryonic stem cells with dental epithelium and dental mesenchyme of wild-type embryos and try to examine the contribution of cells from ESCs to tooth formation.
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| Free Research Field |
歯の発生、器官形成
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| Academic Significance and Societal Importance of the Research Achievements |
我々は、マウス胚性幹細胞株から歯の構成細胞の効率的誘導及び歯の器官形成を目的とし、象牙芽細胞を緑色蛍光でエナメル芽細胞を赤色蛍光で検出できるマウス及び胚性幹細胞株を作成した。我々は初めて象牙芽細胞とエナメル芽細胞を別蛍光で同一個体で検出できる系を確立し、これらを用いて試験管内及び生体内で歯の構成細胞がどのような性質を持ち、どのように歯の器官形成に寄与するかを検討しており、その学術的意義は高い。近年、再生医療の進歩は目ざましいが歯の再生の実用化は進んでいない。義歯、インプラントに加えて歯の再生医療という新たな選択肢が増えることは口腔の機能保全というニーズに大きく関わっており、社会的意義が高い。
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