2017 Fiscal Year Final Research Report
Establishment of iPS cells from McCune-Albright syndrome and it application
| Project/Area Number |
16K15791
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| Research Category |
Grant-in-Aid for Challenging Exploratory Research
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pathobiological dentistry/Dental radiology
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
小野寺 晶子 東京歯科大学, 歯学部, 講師 (90637662)
齋藤 暁子 東京歯科大学, 歯学部, 助教 (90722835)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | McCune-Albright 症候群 / iPS細胞 / GNAS |
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| Outline of Final Research Achievements |
Since distribution of the gene mutated cells in McCune-Albright syndrome (MAS) occurs depending somatic mosaicism, we applied gene editing technique using CRISPR/Cas9 system to generate human iPS cells exhibiting GNAS1 mutation observed in MAS. We obtained 2 clones exhibiting GAS1 mutation among 24 clones. These iPS cells showed undifferentiated markers and those identifying three germ layers. Thus we successfully established human iPS cells retaining GNAS1 mutaion observed in MAS.
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| Free Research Field |
口腔病理学
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