Functional analysis of osteoblastic cell or immature MSC derived exosomes for bone regeneration

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15812
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Nagasaki University
• Principal Investigator: SUMITA Yoshinori 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (50456654)
• Co-Investigator(Kenkyū-buntansha): 朝比奈 泉 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30221039) ; 各務 秀明 松本歯科大学, 総合歯科医学研究所, 教授 (80242866)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 骨再生 / エクソソーム / 分化

Outline of Final Research Achievements

This study aimed to analyze the function of MSC-derived exosomes (MSC-ex) that were harvested from the different stages of differentiation such as immature-, preosteoblastic differentiated-, osteoblastic differentiated-MSCs. In this study, we firstly analyzed the characters of these 3 different MSC-exes, and then evaluated the characteristic changes of MSCs after cultured in medium with these MSC-exes. As results, we found the osteoblastic differentiated-MSC-ex showed the increased expression of osteoblastic genes such as runx2 or alp. Furthermore, we confirmed these MSC-exes are taken into MSCs during 24 hours in culture, and the osteogenic mRNAs were upregulated in MSCs cultured with osteoblastic-differentiated-MSC-ex. In contrast, immature-MSC-ex showed the higher expression levels of mRNAs related to the vasculogenesis such as vegf. We are currently carrying out in vivo analyses for clarifying the actual usefulness of MSC-exes for bone regeneration.

Free Research Field

口腔外科学・再生医学