Approach of production of dental epithelial cells from periodontal ligament mesenchymal cells for tooth regeneration

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15813
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Iwate Medical University
• Principal Investigator: Harada Hidemitsu 岩手医科大学, 歯学部, 教授 (70271210)
• Co-Investigator(Kenkyū-buntansha): 西谷 直之 岩手医科大学, 薬学部, 教授 (10286867) ; 藤原 尚樹 岩手医科大学, 歯学部, 准教授 (20190100) ; 大津 圭史 岩手医科大学, 歯学部, 准教授 (60509066)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ヘルトビッヒ上皮鞘 / 上皮間葉転換 / 歯の再生 / 間葉上皮転換 / セマフォリン

Outline of Final Research Achievements

The aim of this project is to produce dental epithelial stem cells from mesenchymal cells in periodontal ligament by mesenchymal-epithelial transition (MET) and to utilize the epithelial cells for tooth regeneration. In order to obtain their cells, we tried to inhibit the epithelial-mesenchymal transition (EMT) and induce epithelial cells from EMT cells. As a result, Rho activator, Semaphorin4A and 4D, LPA (lysophospholipid) activate Rho signal and inhibited the EMT, and further induced MET and hyper-epithelium. But, it was difficult to obtain enough cell number of dental epithelial cells, it remains as further problem.

Free Research Field

再生歯学