Development of gene-activated matrix with MSC-exosome for bone engineering

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15826
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Surgical dentistry
• Research Institution: Nagasaki University
• Principal Investigator: ASAHINA Izumi 長崎大学, 医歯薬学総合研究科(歯学系), 教授 (30221039)
• Co-Investigator(Kenkyū-buntansha): 住田 吉慶 長崎大学, 医歯薬学総合研究科(歯学系), 准教授 (50456654)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 骨再生 / 遺伝子導入 / エクソソーム / 人工骨

Outline of Final Research Achievements

Therapeutic method for in vivo stem cell or gene delivery has not been established on bone engineering though its potential usefulness has been suggested. In this study, we focused on the esosomes that were derived from osteogenic gene transduced mesenchymal stem cells (MSCs). As experiments, osteogenic MSC-exosomes (MSC-ex) were harvested from cultured mouse bone marrow-MSCs after BMP2/4 gene transfer using plasmid vector. Then, we confirmed the character and the osteogenic induction ability of osteogenic MSC-ex in culture. As results, the expression of BMP2/4 mRNAs in osteogenic MSC-ex was significantly up-regulated, and its osteogenic induction ability was recognized when MSC-ex was added to the MSCs in culture. Therefore, we are currently carrying out in vivo experiments for clarifying the usefulness of GAM combined with osteogenic MSC-ex.

Free Research Field

口腔外科学・再生医学