The involvement of cell polarity in trabecular formation

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15837
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Orthodontics/Pediatric dentistry
• Research Institution: Okayama University
• Principal Investigator: KAMIOKA HIROSHI 岡山大学, 医歯薬学総合研究科, 教授 (80253219)
• Co-Investigator(Kenkyū-buntansha): 坂元 尚哉 首都大学東京, システムデザイン研究科, 准教授 (20361115) ; 早野 暁 岡山大学, 医歯薬学総合研究科, 助教 (20633712)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 細胞極性 / 骨形成

Outline of Final Research Achievements

A knockdown experiment of Nesprin - 1 using siRNA for osteoblast cell line Saos 2 was performed. By introducing Si - Nesprin 1, it was confirmed by immunofluorescence that nuclear localization of Nesprin decreased. Furthermore, it was confirmed that si-Nesprin 1 suppresses expression at the protein level by the Western blotting method. Therefore, it was confirmed that si-Nesprin 1 was introduced into Saos 2 cells.
Next, culture experiments of knockdown cells using uneven surface structures were performed. As a result, there was no significant difference between the two in the arrangement of the cells. Next, in order to examine the change of Saos 2 in cell polarity in mechanical stress, cell extension experiment with flexer cell unit was performed. However, in Saos 2, no change in polarity to mechanical stress was observed, so Nesprin's involvement was not considered.

Free Research Field

歯科矯正学