Feasible research for novel periodontal regeneration therapeutics by CRISPR/Cas9

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15846
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Periodontology
• Research Institution: Tohoku University (2017); Osaka University (2016)
• Principal Investigator: YAMADA Satoru 東北大学, 歯学研究科, 教授 (40359849)
• Co-Investigator(Kenkyū-buntansha): 竹立 匡秀 大阪大学, 歯学部附属病院, 講師 (60452447) ; 山下 元三 大阪大学, 歯学部附属病院, 講師 (90524984)
• Research Collaborator: KINOSHITA Masaki 大阪大学, 大学院歯学研究科, 大学院生
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 歯根膜 / ゲノム編集 / CRISPR/Cas9

Outline of Final Research Achievements

In order to develop novel periodontal regeneration therapeutics, we tried to develop genome editing in periodontal ligament cells using CRISPR/Cas9 system. First, we selected PLAP-1, Decorin and Biglycan genes as targets for CRISPR/Cas9 mediated gene editing. We constructed specific sgRNA-CRISPR/Cas9 expression plasmid and performed gene transfection into MPDL22 cells. Since gene transfer efficiency was not good enough for gene editing in MPDL22 cells, we are currently trying to establish primary periodontal ligament cells from PLAP-1 KO mice. The primary PLAP-1 KO cells will be helpful to edit single genomic locus of Decorin or Biglycan by CRISPR/Cas9.

Free Research Field

歯周病学