The model of gingival sulcus made from Revival gums: the pre-clinical study for an animal experiment

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K15858
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Multi-year Fund
• Research Field: Social dentistry
• Research Institution: The Nippon Dental University
• Principal Investigator: Yaegaki Ken 日本歯科大学, 生命歯学部, 教授 (40166468)
• Co-Investigator(Kenkyū-buntansha): 田中 とも子 日本歯科大学, 生命歯学部, 准教授 (70307958)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: バイオフィルム / 歯肉溝 / 歯周病

Outline of Final Research Achievements

The purpose of this study is how to establish a gingival sulcus model by reclaimed gums and to drive the pre-clinical study.
The microorganisms were Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Streptococcus gordonii. We tried biofilm formation on the section of a human tooth with the TYGVS culture medium added N-acetylmuramic acid. The biofilm formation was confirmed by the SEM observation and the bacterial flora analyses.
And the stem cells segregation became a simple method by the magnetic separation. But reproduction of an artificial gingival sulcus didn't come to realization.

Free Research Field

口腔衛生学