Genome instability induced by high-energy phosphate compounds

2019 Fiscal Year Final Research Report

• Project/Area Number: 16K16195
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Chiba University
• Principal Investigator: Sassa Akira 千葉大学, 大学院理学研究院, 特任助教 (10738347)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: DNA修復 / リボヌクレオチド / 損傷乗り越えDNA合成 / ゲノム不安定性

Outline of Final Research Achievements

Ribonucleotides are essential for cellular metabolism and energy production. On the other hand, ribonucleotides are often incorporated into the genome by DNA polymerases in mammalian cells. The aberrant accumulation of ribonucleotides in the genome causes cellular abnormalities. In this study, we have clarified the mutagenic potential of a ribonucleotide incorporated into DNA and possible alternate repair pathways to suppress the mutagenic effect of an embedded ribonucleotide in human cells.

Free Research Field

化学物質影響関連

Academic Significance and Societal Importance of the Research Achievements

リボヌクレオチドのゲノムへの蓄積は、重度心身障害をもたらすAicardi-Goutieres症候群（AGS）の発症や細胞のがん化と深く関連している考えられる。DNAに取り込まれたリボヌクレオチドが誘発するゲノム不安定化とその抑制因子を明らかにすることで、疾患の発症メカニズムならびに生物の新たなゲノム制御機構の解明につながる。