Elucidate of change mechanism of harmful algal blooms based on the novel GWGS (genotyping by whole genome sequencing) method.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K16206
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental engineering and reduction of environmental burden
• Research Institution: Akita Prefectural University
• Principal Investigator: Okano Kunihiro 秋田県立大学, 生物資源科学部, 助教 (30455927)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 有毒アオコ / 八郎湖 / ホールゲノム解析 / 次世代シーケンサー

Outline of Final Research Achievements

With the ultimate goal of clarifying the change mechanism of harmful algal blooms in the Lake Hachiro (Akita Prefecture), Microcystis sp. isolated from Lake Hachiro were classified by the novel whole genome genotyping (GWGS) method and the growth characteristics of Microcystis sp. was investigated. The low temperature-adapted Microcystis sp. strain 0824 had a characteristic gene structure, and the homology of strain 0824 to Microcystis sp. (10 strains) analyzed by GWGS was at most 73.7%. In addition, it was clarified that Microcystis sp. strain 0824 was non-microcystin-producing strain with mcy gene cluster deletion and strain N7 was microcystin-producing strain with mcy gene cluster conservation.

Free Research Field

微生物生態学