Development of autofluorescence-free Raman spectroscopy and its applications to biomedical imaging

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K16389
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: The University of Tokushima
• Principal Investigator: Minamikawa Takeo 徳島大学, 大学院社会産業理工学研究部(理工学域), 講師 (10637193)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ラマン散乱分光法 / ラマン散乱 / 自家蛍光 / ラマン顕微鏡

Outline of Final Research Achievements

Raman spectroscopy is a promising technique to visualize molecular information of cells and tissues based on molecular vibrations. This is now widely applied to biomedical imaging and its molecular analysis. However, autofluorescence from specimens limits the sensitivity of Raman spectroscopic analysis due to the autofluorescence spectrum appears at the same wavelength of the Raman spectrum and works as a strong background. In this study, we developed a Raman spectroscopy system with an autofluroescence elimination system based on the excitation wavelength dependency of the Raman scattering and autofluroescence.

Free Research Field

顕微分光学