2016 Fiscal Year Research-status Report
Development of controllable nitric oxide-releasing injectable hydrogel with ROS scavenging effect for biomedical therapies
| Project/Area Number |
16K16397
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Vong BinhLong 筑波大学, 数理物質系, 研究員 (40771960)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | nitric oxide / reactive oxygen species / redox nanoparticle / redox injectable gel / cardiovascular disease / angiogenesis |
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| Outline of Annual Research Achievements |
In this study, we developed an redox injectable hydrogel (NO-RIG), which generates nitric oxide (NO) and scavenges reactive oxygen species (ROS), for cardiovascular disease.
In our experiment, we successfully prepared and analyzed NO-RIG and other control injectable gels. We also performed myocardial infarction (MI) model mice and confirmed the therapeutic effects of NO-RIG on this model as compared to other control gel.
We could also confirmed the disintegration of gel in vitro and retention of gel in vivo after intracardial injection.
Using echocardiogram, cardiac functions were evaluated after MI and injection of gels. We foundt that NO-RIG showed significant therapeutic efficacy in MI. Interestingly, we could investigate the angiogenesis and the generation of NO in vivo mice.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
This work is very interesting with high originality. We try our effort to investigate the therapeutic effect and mechanism underlying.
Because we used very high level of animal model such as myocardial infarction models mice or angiogenesis model in vivo, which are not easy to perform properly.
Fortunately, we collaborated experts who has long experience on these animal models. And our research teams are always supported by university and collaborators
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| Strategy for Future Research Activity |
- We further investigate the mechanism of Nitric oxide release from NO-RIG under infarction areas
- We are going to confirm the potential toxicity of our developed gels in vitro and in vivo systems
- We also investigate the ROS scavenging and NO release in vivo.
- We will prepare the manuscript for publication in the Scientific Journal with high quality
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