2017 Fiscal Year Annual Research Report
Development of controllable nitric oxide-releasing injectable hydrogel with ROS scavenging effect for biomedical therapies
| Project/Area Number |
16K16397
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| Research Institution | University of Tsukuba |
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Principal Investigator |
Vong BinhLong 筑波大学, 数理物質系, 研究員 (40771960)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | injectable hydrogel / myocardial infarction / nitric oxide |
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| Outline of Annual Research Achievements |
Research Summary: Nitric oxide (NO) possesses various functions in cardiovascular diseases; however, due to an extremely short half-life and low bioavailability, its therapeutic application is limited. In inflamed tissues, overproduced reactive oxygen species (ROS) rapidly react with the endogenous NO, reducing its bioavailability. Here, we developed a controllable NO-releasing injectable hydrogel (NO-RIG) formed by the electrostatic irreversibly crosslinking between the polyion complex flower-type micelles composing of functional polymers to scavenge overproduced ROS and regulate the local NO expression level simultaneously. After the intracardiac injection to mice, NO-RIG converted to gel via physiological temperature-responsive character, distributed homogeneously, and retained in the myocardial tissue for more than 10 d. Treatment with NO-RIG remarkably decreased the infarction size and improved the heart function after myocardial infarction when compared to control injectable hydrogels, such as a simple NO-releasing or ROS-scavenging injectable gels. We found that NO-RIG treatment significantly enhanced the angiogenesis and new blood vessels formation in mice through the regulation of the NO sustained release and redox equilibrium. NO-RIG presents high potential in preventing and treating cardiovascular diseases.
- This work was published in Biomaterials and presented in Polymer meeting, Gelsymposium, American chemistry Society Meeting.
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