Supermolecule-mediated growth factor-immobilized surfaces for regulating cellular functions

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K16399
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomedical engineering/Biomaterial science and engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ARISAKA Yoshinori 東京医科歯科大学, 生体材料工学研究所, 助教 (70590115)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: ポリロタキサン / 成長因子

Outline of Final Research Achievements

We prepared growth factor-immobilized polyrotaxane (PRX) surfaces and tried to regulate cellular functions by surface-immobilizing growth factor and modulating surface molecular mobility. Previously, it has been reported that sulfated PRXs form complexes with bone morphogenetic proteins (BMP-2). In the present study, sulfated PRX surfaces with immobilized BMP-2 were designed. It was revealed that the combination of surface-immobilization of BMP-2 and low molecular mobility of PRXs significantly promotes osteoblast differentiation of preosteoblasts. This suggests that regulation of molecular mobility and surface-immobilization of BMP-2 synergistically enhanced osteoblast differentiation. Similarly, it was revealed that the PRX surface is also useful in controlling the cellular functions of mesenchymal stem cells, liver-derived cells, vascular endothelial cells.

Free Research Field

バイオマテリアル科学

Academic Significance and Societal Importance of the Research Achievements

環状分子と線状高分子とからなるポリロタキサンを被膜した表面(ポリロタキサン表面)において、ポリロタキサン骨格に由来する表面分子可動性によって接着細胞の機能が調節できることを報告している。本研究課題では、このようなポリロタキサン表面と成長因子をインテグレーションすることによって、さらに高度な細胞機能操作が可能となることを明らかにした。