Novel chemical protein labeling approach for analyzing the intracellular cargo transport system in kinesin

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K16635
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biomolecular chemistry
• Research Institution: Hokkaido University
• Principal Investigator: Matsuo Kazuya 北海道大学, 電子科学研究所, 助教 (90764952)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: キネシン / 微小管 / モータータンパク質 / 化学修飾

Outline of Final Research Achievements

Kinesin is one of motor proteins, which is deeply involved in the important biophenomena including the intracellular transportation of cargo stuffs (organelles and vesicles). In this study, we developed the chemical methods for labeling the kinesin-specific protein-protein interaction to reveal the cargo systems in kinesin. The candidate of the new reactive handle which can change the reactivity based on redox conditions was successfully discovered. We also developed the light-switchable inhibitor to control the kinesin motility. Using this inhibitor, the light controllable kinesin was chemically constructed.

Free Research Field

生物有機化学