Understanding hydraiton-water dynamics related to amyloid fibril formation

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K17783
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological physics/Chemical physics/Soft matter physics
• Research Institution: Kobe University
• Principal Investigator: Yamamoto Naoki 神戸大学, 理学研究科, 特命助教 (90580671)
• Research Collaborator: TOMINAGA Keisuke 神戸大学, 分子フォトサイエンス研究センター, 教授 (30202203) ; CHATANI Eri 神戸大学, 大学院理学研究科, 准教授 (00432493) ; NAKANISHI Masahiko 福岡工業大学, 電気工学科, 助教 (00707763)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 広帯域誘電分光 / タンパク質の水和水 / アミロイド線維 / 線維前駆中間体 / 回転緩和

Outline of Final Research Achievements

Protein misfolding sometimes results in formation of abnormal aggregation, or amyloid fibril, which are thought to be causes of specified diseases such as generalized amyloidoses. In order to understand the underlying mechanism of the amyloid fibril formation, we scrutinized the dynamics of water around proteins or amyloid fibrils, i.e. hydration water, at gigahertz and terahertz regions. We performed temperature-dependent broadband dielectric spectroscopy in the frequency region by obtaining the complex dielectric spectra. We found that the rotational relaxational mode of hydration water became faster in the fibril state compared to the monomeric normal state while the relaxation time was similar between a prefibrillar intermedaite state and the fibril state.

Free Research Field

生物物理化学