Observation of three-dimensional structural dynamics in biological chemical reactions based on Raman optical activity

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K17859
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical chemistry
• Research Institution: Saga University
• Principal Investigator: Fujisawa Tomotsumi 佐賀大学, 工学(系)研究科(研究院), 特任講師 (60633493)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ラマン光学活性 / 光受容タンパク質 / 反応中間体

Outline of Final Research Achievements

This　research　have been aiming to develop Raman optical acitivity (ROA) spectroscopy to track　the　three-dimensional (3D)　structure　of　the　reaction　intermediates in photoreceptor proteins. We successfully measured the ROA spectrum for the transient state of orange cartenoid protein and analysed the 3D structure. We also built the low-temperature Raman setup to measure the photo-itnermediates of various photoreceptors . We obtained good Raman data for the photo-intermediates of a couple of photoreceptors, which allows us to apply ROA spectroscopy to the photointermediates.

Free Research Field

物理化学