2017 Fiscal Year Final Research Report
Design of a novel affinity labeling probe exhibiting fluorescence and luminescence
| Project/Area Number |
16K17930
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bio-related chemistry
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| Research Institution | Osaka University (2017)
The University of Tokyo (2016) |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | アフィニティーラベル化 / 標的タンパク質同定 / 発蛍光プローブ分子 / 創薬化学 |
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| Outline of Final Research Achievements |
To elucidate the mechanism of bioactive small molecules, target protein identification methods are highly important. In this project, a novel affinity labeling probe having azidophthalimide structure was designed. By several model experiments, we revealed that the designed probe is suitable for selective fluorescent labeling of the target protein. Furthermore, the ligand-binding site was successfully determined by this method. We also successfully designed and synthesized several bioactive small molecules.
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| Free Research Field |
有機合成化学、生物有機化学、ケミカルバイオロジー、創薬化学
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