The effect of payload conjugates on VHH antibody

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K17939
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Bio-related chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Akazawa Yoko 国立研究開発法人産業技術総合研究所, バイオメディカル研究部門, 主任研究員 (50549897)
• Research Collaborator: Hagihara Yoshihis 国立研究開発法人産業技術総合研究所 (50357761) ; Nakajima Yoshihiro 国立研究開発法人産業技術総合研究所 (10291080)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: シングルドメイン抗体 / 異分子コンジュゲート / 抗体ー薬物複合体

Outline of Final Research Achievements

Developing the technique to conjugate antibody with various payload reagents, such as anticancer drug, isotopes and dye, is an important topic for evolution of antibody drug, diagnostic reagent and molecular imaging. The technical difficulty for the homogeneous conjugation to conventional IgG and IgG-derived fragments is caused by inevitable features of antibody structure. Single-domain VHH antibody, variable domain of camelid heavy chain antibody, usually has only one disulfide bond and thus is useful to obtain a homogeneous population with a fixed conjugation at a specific location. Here we report the feasibility of payload (Low molecular weight compounds, nucleic acids) conjugation with VHH antibody. The conjugate was then labeled, and its stability and functionality were characterized.

Free Research Field

蛋白質工学