Two photon calcium imaging of layer 6 neurons in primary motor cortex

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18370
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Neurophysiology / General neuroscience
• Research Institution: The University of Tokyo
• Principal Investigator: Tanaka Yasuhiro 東京大学, 大学院医学系研究科(医学部), 助教 (20533128)
• Research Collaborator: TANAKA Yasuyo
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 6層 / 大脳皮質 / 2光子カルシウムイメージング

Outline of Final Research Achievements

The functional significance of layer 6, the deepest layer, of the neocortex in animal behaviors is poorly understood because the recordings of neurons in layer 6 was rather limited in behaving animals. We performed two photon calcium imaging of layer 6 in the primary motor cortex of behaving mice. We established stable recordings of multiple single neurons in layer 6 which were located ~800-micrometer deep from the brain surface, by using RCaMP 1.07, a genetically encoded calcium indicator. Throughout this research period, I wrote various scripts for image acquisition and motion correction, which were used in some published papers.

Free Research Field

大脳皮質機能学