Comprehensive analyses of controlling mechanism for higher-order structure of chromatin and gene transcription regulation mediated by Poly-ADP-ribosylation

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K18469
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: The University of Tokyo
• Principal Investigator: Fujiki Katsunori 東京大学, 定量生命科学研究所, 助教 (10646730)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: PAR化 / 次世代シーケンサー / １細胞解析 / ChIA-drop

Outline of Final Research Achievements

Protein Poly-ADP-ribosylation in cell nuclear drastically changes surrounding chromatin environment by its electric property, and regulates many biological process including gene transcription and DNA repair. In this project, I developed a new research method to detect the change of higher-order chromatin structure by this modification. ChIA-drop, the new method which was named on its use of DNA labeling technology in micro droplet, is the first research method that enables detection of "multi-contact" chromatin interaction in "single-molecule" resolution, and provides advantages over the existing techniques including Hi-C and ChIA-PET that base on accumulation of pairwise-contact information of many molecules.

Free Research Field

分子生物学

Academic Significance and Societal Importance of the Research Achievements

本研究で開発したChIA-drop法を用いることで、PAR化修飾によるクロマチン高次構造制御機構の理解がさらに深まることが期待できる。またこのChIA-drop法はPAR化のみを標的とした解析手法にとどまらず、インプットとして用いるサンプルを変えることで、様々な標的タンパク質を介したクロマチン高次構造の解析に用いることが可能である。ChIA-drop法の多点間のクロマチン相互作用を１分子の解像度で解析できるという特徴は、既存の方法では実現できなかった利点であり、この手法を用いることで広くクロマチン高次構造の研究が進展すると考えられる。