Development of screening method using large deletion in Marchantia polymorpha

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18472
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Genome biology
• Research Institution: Ritsumeikan University (2017); Kyoto University (2016)
• Principal Investigator: Sugano Shigeo 立命館大学, 立命館グローバル・イノベーション研究機構, 助教 (60726313)
• Co-Investigator(Renkei-kenkyūsha): Kohchi Takayuki 京都大学, 生命科学研究科, 教授 (40202056) ; Katsuyuki Yamato 近畿大学, 生物理工学部 生物工学科, 教授 (50293915)
• Research Collaborator: Koumoto Yasuko 京都大学, 理学研究科, 教務補佐
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ゼニゴケ / Marchantia polymorpha / ゲノム編集 / 染色体

Outline of Final Research Achievements

Genome editing vectors for large deletion in Marchantia polymorpha were constructed. Using these vectors, the transformants harboring the 4.5 kbp deletion were obtained at the rate of 25% of the T1 transformants. The vectors were deposited and the research paper about the efficient genome editing in M. polymorpha was open at preprint server. In addition, we implemented the software that can automatically design gRNAs for all the gene, including sex chromosomes, in M. polymorpha. The presentation about the software was done at the international meeting.

Free Research Field

ゲノム生物学