2017 Fiscal Year Final Research Report
Molecular mechanism elucidation of multifunctional tRNA ligase in tRNA splicing
| Project/Area Number |
16K18498
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
Kato Koji 北海道大学, 先端生命科学研究院, 助教 (30452428)
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| Research Collaborator |
SAKURAI Naofumi
SUZUKI Wakana
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | tRNAスプライシング / X線結晶構造解析 |
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| Outline of Final Research Achievements |
Crystal structure of Trl1 ligase domain (Trl1-LD) - GMP complex was determined with 2.75 angstrom resolution. Two molecules of GMP were stacked and bonded near the active center of Trl1-LD. As a result of detailed analysis of the complex structure, basic amino acids were concentrated around two molecules of GMPs. From these facts, it was considered that these GMPs are mimicking tRNA exons in the ligation process. Based on this structure, it was possible to infer the binding mode of tRNA of Trl1-LD.
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| Free Research Field |
構造生物学
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