2017 Fiscal Year Final Research Report
Structural study on the NKRP1A-LLT1 complex involved in the immune regulatory
| Project/Area Number |
16K18499
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Structural biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 免役受容体 / 相互作用 / 構造解析 |
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| Outline of Final Research Achievements |
Human NKRP1A is an immunoreceptor that highly expressed in NK cells and Th17 cells, and is a member of the immunity checkpoint receptor which is attractive for a cancer drug discovery target in recent years. In this study, we aimed to clarify the interaction between NKRP1A and its ligand, LLT1, by physicochemical and structural biology and to elucidate the molecular basis of immune regulatory mechanism involving NKRP1A. An extensive mutagenesis at the interaction surface inferred from the NKRP1A-LLT1 complex model and binding analysis was carried out. The results suggested that the interaction might be precisely regulated to the extent that even a slight difference in amino acid side chain structure at the interacting surface is unacceptable.
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| Free Research Field |
タンパク質工学、生物物理化学
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