Improving diffraction resolution of protein crystal by high pressure

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18502
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Structural biochemistry
• Research Institution: Nagoya University
• Principal Investigator: Nagae Takayuki 名古屋大学, シンクロトロン光研究センター, 特任助教 (90735771)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 圧力 / 水和 / 分解能

Outline of Final Research Achievements

In this study we researched relationship between diffraction resolution of protein crystals and high pressure. For several kinds of proteins, diffraction data of crystals were collected and analyzed. High pressure tended to improve diffraction resolution of proteins which have low molecular weight. The reason is considered that high pressure makes hydration structure ordered and as a result diffraction ability of entire crystal improve. High pressure could improve crystal resolution of proteins which have low molecular weight, regardless of kinds of proteins or crystallization conditions.

Free Research Field

蛋白質結晶構造解析