2016 Fiscal Year Research-status Report
A realistic mechano-sensitive disease model in-vitro for cardiac tissue culture.
| Project/Area Number |
16K18525
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| Research Institution | Kyoto University |
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Principal Investigator |
Hoerning Marcel 京都大学, 物質－細胞統合システム拠点, 客員研究員 (20588496)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | Hydrogel / Mechanosensing / C2C12 |
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| Outline of Annual Research Achievements |
The first step of the study is successfully finished. We setup the experimental system using Host-Guest gels and C2C12 myocytes. We studied the general behavior and responds of the gel and the mechxnosensitive behavior of the cells. The study is currently summarized toward the successful publication. The tentative title is "Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions".
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
The study is currently summarized toward the successful publication. The tentative title is "Dynamic Mechano-Regulation of Myoblast Cells on Supramolecular Hydrogels Cross-Linked by Reversible Host-Guest Interactions".
Currently we setup the primary cardiac tissue culture to study the mechanosensitive process and responses, as proposed in the outline.
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| Strategy for Future Research Activity |
We will setup the culture setup of in vitro observation system with primary cardiac tissue and investigate the developmental processes and wave excitation behavior of the confluent tissue under mechanical stimuli.
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| Causes of Carryover |
Up to 30% of the money will be used for travel expenses from and to Japan and Germany, respectively, since I will be located in Germany. The cell culture of neonatal rats needs to be setup and maintained, as well as fluorescence probes need to be used. In parallel C2C12 cell line needs to be maintained.
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| Expenditure Plan for Carryover Budget |
travel and accommodation, animal model: neonatal Wistar rats, median, fluorescence probes, glass ware etc.
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