2017 Fiscal Year Research-status Report
A realistic mechano-sensitive disease model in-vitro for cardiac tissue culture.
| Project/Area Number |
16K18525
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | Hydrogel / Mechanosensing / C2C12 |
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| Outline of Annual Research Achievements |
We successfully published two works that are related to the current project. The first paper deals with the dynamics of the reversible dynamics of the hydrogel and its regulatory behavior to cells (Hoerning et al, Scientific Reports, 7, 7760, 2017). The supramolecular hydrogel is cross-linked by host-guest interactions between β-cyclodextrin (βCD) and adamantane, and were designed for the dynamic regulation of cell-substrate interactions. The magnitude of softening and stiffening of the substrate can be modulated by varying the concentrations of free, competing host molecules (βCD) in solutions. By changing the substrate elasticity at a desired time point, it is possible to switch the micromechanical environments of cells. Here, we use C2C12 muscle cells as a simple biological system to test the dynamics and response dynamics of the hydrogel to the cells. The second paper deals with cardiac dynamics. In particular we developed a novel method to extract and quantify the dynamics of line defects (Hoerning et al, Scientific Reports, 7, 7757, 2017). Line defects spatial separate electrophysiological oscillations in the tissue and are known to be a precursor of deceased states in heart, thus having potential to induce life-threatening fibrillation. In this study we show that line defects can translate, merge, collapse and form stable singularities with even and odd parity while maintaining a stable oscillation of the spiral wave in the tissue.
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| Current Status of Research Progress |
Current Status of Research Progress
1: Research has progressed more than it was originally planned.
Reason
We have obtained new more complex dynamics of C2C12 cells on glass substrates to develop a more sophisticated method that can extract the membrane dynamics in 3D. A study that investigated the 3D dynamics of membrane proteins in currently under submission and was the first step to achieve the mentioned goal.
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| Strategy for Future Research Activity |
Though our study progress is more smoothly than initially planned. We added more detailed steps and research foci in order to obtain a larger impact of our findings in future. So we stay on track with the initial research plan but added some sub-investigations that will enhance the scientific quality.
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| Causes of Carryover |
Our results that we published so far are very promising and leading to even more important and interesting scientific questions. We believe that the money planed to used for the next fiscal year is well spend and necessary to proceed this study.
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