2018 Fiscal Year Final Research Report
A realistic mechano-sensitive disease model in-vitro for cardiac tissue culture.
| Project/Area Number |
16K18525
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biophysics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Research Collaborator |
Shibata Tatuso
Harada Akira
Takashima Yoshinori
Nakahata Masaki
Tanaka Motomu
Umeshima Hiroki
Kengaku Mineko
Isomura Akihiro
Blanchard Francois
Yoshikawa Kenichi
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Keywords | Cardiac Tissue / Cellmechanics / Patternformation |
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| Outline of Final Research Achievements |
We revealed novel insides of the regulation of cell morphology and cytoskeletal ordering of myoblasts without interfering cell viability by using novel dynamically changeable hydrogels. Further, we introduced a novel analysis method to visualise spatiotemporal dynamics of line defects in rotating spiral waves of cardiac tissues, showing that line defects can translate, merge, collapse and form stable singularities while maintaining a stable oscillation of the spiral wave. Additionally, we introduced a novel mapping method to quantify lipid dynamics on the entire cell membrane of single cells. We revealed that PtdInsP3 wave dynamics are directly regulated by the 3D geometry (i.e., size and shape).
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| Free Research Field |
Biology, Physics
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| Academic Significance and Societal Importance of the Research Achievements |
Main goal of this investigation is to understand tissue morphological changes and pattern formation that are associated with cardiac diseases, which is in JAPAN one of the leading causes of death with an annual death rate of approx. 100,000 people (12%).
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