The analysis of the role of SOX9 post-translational modification in vertebrate skeletal development

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18558
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Developmental biology
• Research Institution: Meiji University (2017); National Center for Child Health and Development (2016)
• Principal Investigator: Inui Masafumi 明治大学, 農学部, 専任講師 (20643498)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: SOX9 / 翻訳後修飾 / 軟骨 / ゲノム編集

Outline of Final Research Achievements

SOX9 is the master regulator of cartilage formation and its quantitatively precise activity is crucial for the reproducible skeletal development. In this study, we focused on the protein post-translational modification on SOX9, especially SUMOylation on K396, and generated the point mutation mouse carrying the K396R substitution and thus devoid of SUMOylation on this residue. SOX9K396R mouse showed reduced body weight and length with skeletal abnormality, which imply the importance of K396 SUMOylation on reproducible skeletal development. Molecularly, SUMO-SOX9 showed repressive activity on wild type SOX9. Further study will be necessary to identify the associating protein(s) that could account for this activity.

Free Research Field

発生生物学