Functional analysis of the role of plant tyrosine protien kinase for gibberellin signalling.

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18570
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Iwate Biotechnology Research Center (2017); Ehime University (2016)
• Principal Investigator: Nemoto Keiichiro 公益財団法人岩手生物工学研究センター, 園芸資源研究部, 主任研究員 (60566727)
• Co-Investigator(Renkei-kenkyūsha): SAWASAKI Tatsuya 愛媛大学, プロテオサイエンスセンター, 教授 (50314969) ; TOMII Kentaro 国立研究開発法人産業技術総合研究所, 情報・人間工学領域, 研究チーム長 (40357570) ; IMAI Kenichiro 国立研究開発法人産業技術総合研究所, 研究員 (80442573) ; ARIMURA Genichiro 東京理科大学, 基礎工学部, 准教授 (60505329)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: リン酸化 / ユビキチン化 / ジベレリン / シグナル伝達

Outline of Final Research Achievements

Several recent studies suggest that plants have a Tyr-phosphorylation signal pathway, although, the role of Tyr-phosphorylation in biochemical and physiological processes is poorly understood. Here, we demonstrate that GARU (GA receptor RING E3 ubiquitin ligase) mediates ubiquitin-dependent degradation of phytohormone gibberellin receptor GID1, and that the plant tyrosine-protein kinase TAGK2 inhibits GARU-GID1A interactions by phosphorylation of GARU at Tyr residue. We propose that GA response is negatively regulated by GARU-dependent GID1 ubiquitination and positively by Tyr phosphorylation of GARU by TAGK2.

Free Research Field

生化学