2017 Fiscal Year Annual Research Report
Comprehensive identification of cis-regulatory elements involved in the evolutionary origin of the turtle carapace
| Project/Area Number |
16K18595
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
PASCUAL JUAN 国立研究開発法人理化学研究所, 倉谷形態進化研究室, 研究員 (30594098)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 比較ゲノム / 形態進化 |
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| Outline of Annual Research Achievements |
First, we completed the sequencing of the ATAC-seq libraries for each of the tissues analyzed, for two biological replicates. In total, we have generated 1,456 Million reads (PE100), amounting to a total length of 142 Gbp of clean data. Then, in order to gain a better resolution of the open chromatine regions, I have made high-quality, custom, polyclonal antibodies to perform ChIP-seq analyses against of two different factors of the Wnt pathway of the turtle Pelodiscus sinensis: LEF-1, a transcription factor (TF) active in the carapacial ridge, and β-catenin, a co-factor essential for the canonical Wnt pathway. We have performed ChIP experiments using the same tissues as in the ATAC-seq and the previous ChIP-seq for histone modifications.
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