Identification and functional analysis of loci involved in transformation amenability in barley

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K18634
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Science in genetics and breeding
• Research Institution: Okayama University
• Principal Investigator: Hisano Hiroshi 岡山大学, 資源植物科学研究所, 准教授 (70415454)
• Research Collaborator: SATO Kazuhiro 岡大植物研 ; MUNEMORI Hiromi 岡大植物研 ; SHINKAI Norio 産総研 ; SESE Jun 産総研 ; TOYODA Atsushi 遺伝研 ; SEKI Masahide 東大新領域 ; SUZUKI Yutaka 東大新領域
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: オオムギ / 形質転換 / 再分化 / カルス化

Outline of Final Research Achievements

In this study, we tried to narrow down the regions of TFA (Transformation amenability) loci involved in transformation efficiency in barley in order to enable genetic transformation without limitation of their genotypes. First, SNPs array analysis and exome-sequence analysis were performed to develop the selection markers for TFAs. These markers were used to generate the near-isogenic lines of TFAs derived from a cross between barley cultivar “Haruna Nijo” and “Golden Promise” (GP). Subsequently, the transformation experiment was carried out using the doubled-haploid lines derived from a cross between barley cultivar "Full Pint" and GP to clarify that TFAs were necessary for barley transformation.

Free Research Field

植物分子育種学

Academic Significance and Societal Importance of the Research Achievements

本研究では、オオムギの形質転換効率に関わる遺伝子座TFAの機能の確認、領域の絞り込みおよび領域内に存在する発現遺伝子の確認をすることができた。今後、TFAの原因遺伝子を単離して、それらを制御することにより、多くのオオムギ品種で形質転換が可能となる。このことにより、効率よくオオムギの有用遺伝子の機能解析が可能となり、植物育種への利用も期待できる。本成果は、ゲノム編集等のオオムギ遺伝子改変技術への応用のみならず、他の近縁作物へ適用可能と考えられ、植物科学全体の発展に貢献するであろう。