2017 Fiscal Year Final Research Report
RNAi in spider mites based on noninvasive delivery of dsRNA
| Project/Area Number |
16K18661
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant protection science
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| Research Institution | Tokyo University of Agriculture and Technology |
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Principal Investigator |
Suzuki Takeshi 東京農工大学, (連合)農学研究科(研究院), 特任准教授 (60708311)
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| Research Collaborator |
Grbic Miodrag
Grbic Vojislava
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | RNAi / RNA農薬 / 経口投与 / ハダニ |
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| Outline of Final Research Achievements |
RNA interference (RNAi) has been widely used for the gene functional analysis in many organisms. Double-stranded RNA (dsRNA) that triggers the RNAi-mediated gene silencing has a potential for controlling a targeted pest species as a next generation of pesticides because of its sequence-specific manner. To assess the potential of RNAi-based pest control approaches in the two-spotted spider mite, Tetranychus urticae, two methods for the delivery of dsRNA were developed. These methods are based on mite feeding on dsRNA-coated leaves and mite soaking in a dsRNA solution.The gene targeted for method validation was the Vacuolar-type H+-ATPase (TuVATPase) encoding an ATP-driven proton pump located in the tonoplast. In both methods, RNAi induced down-regulation of TuVATPase, mortality and the reduction of fecundity. These methods will lead to large-scale RNAi screens as a first step towards the development of T. urticae-specific RNA pesticides.
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| Free Research Field |
応用昆虫学
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