Identification and analysis of genes involved in the regulation of aerobic glycerol metabolism of lactic acid bacteria

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18681
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Okayama University of Science
• Principal Investigator: Doi Yuki 岡山理科大学, 工学部, 研究員 (20705412)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 乳酸菌 / Enterococcus faecalis / グリセロール代謝 / トランスポゾン / 糖新生

Outline of Final Research Achievements

The expression of genes encoding the enzymes required for glycerol metabolism of the lactic acid bacterium Enterococcus faecalis is induced by glycerol or glycerol and oxygen; however, the genes involved in the regulation of glycerol metabolism are yet to be identified.
In the present study, we performed in vitro transposon mutagenesis of E. faecalis and identified several genes required for aerobic glycerol metabolism. Experiments using these transposon mutants indicated that the supply of ribose synthesized via gluconeogenesis is essential for nucleic acid biosynthesis in aerobic glycerol metabolism of E. faecalis.

Free Research Field

応用微生物学