D-amino acid biological system in eukaryote

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18686
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Nagoya University
• Principal Investigator: Ito Tomokazu 名古屋大学, 生命農学研究科, 講師 (90584970)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: D-セリン / D-アスパラギン酸 / D-アミノ酸

Outline of Final Research Achievements

We analyzed the catalytic and regulatory mechanisms of D-Asp synthetic activity of mammalian serine racemase (SR). Data inidicaed that SR is one of mammalian D-Asp biosynthetic enzymes. In addition, we analyzed urinary D-Ser using our D-, and L-Ser enzymatic assay method. The results obtained here suggested that D-Ser levels could be used as a predictable biomarker of renal function reduction in some situations. In addition, we studied the molecular mechanism of developmental abnormalities of Dsd-deficient Dicsyotelium strains and revealed the involvment of D-Ser in cAMP signaling. We found a novel Dsd substrate D-amino acid in the fruiting body of Bunashimeji mushroom, and identified it as (2R, 3S) 2-amino-3,4-dihydroxybutanoic acid.

Free Research Field

応用生物化学