2017 Fiscal Year Final Research Report
Development of highly functionalized cell culture substratum with ganglioside glycans
| Project/Area Number |
16K18694
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioorganic chemistry
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| Research Institution | Gifu University |
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Principal Investigator |
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| Research Collaborator |
MUTO Tatsuro
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | フィブロイン / ガングリオシド / 糖鎖修飾 / バイオマテリアル |
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| Outline of Final Research Achievements |
We have developed a chemical modification method for introducing highly bioactive gangliosides to silk fibroin protein, which has been used as a matrix material in the field of tissue engineering. As a glycan to modify silk fibroin, the glycan part of echinodermatous ganglioside LLG-3 was chosen due to a high degree of the neurogenic activity and chemically synthesized. The synthetic LLG-3 glycan with a linker bearing an aromatic amine at the terminal was coupled with tyrosine residues in silk fibroin via diazonium coupling reaction, leading to the generation of ganglioside-modified silk fibroin for the first time.
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| Free Research Field |
応用糖質化学
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