Integration of transcriptomic information during ovarian maturation process in Penaeid shrimps

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18746
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic bioproduction science
• Research Institution: Japan International Research Center for Agricultural Sciences
• Principal Investigator: Okutsu Tomoyuki 国立研究開発法人国際農林水産業研究センター, その他部局等, 研究員(移行) (40456322)
• Research Collaborator: MARUYAMA Kyonoshin 国際農林水産業研究センター, 水産領域, 主任研究員
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: バナメイエビ / 卵巣成熟 / 分子マーカー / マイクロアレイ / クルマエビ科

Outline of Final Research Achievements

We found 66,166 genes encoding a certain protein among 72,348 unigene sequences in whiteleg shrimp, Litopenaeus vannamei, obtained from NCBI database, and revealed their amino acid sequences. The microarray probes were designed from the above-mentioned 72,348 unigene sequences using eArray software. A comparative transcriptomic analysis using the array revealed GILT and NP1 gene showed specific expression in mature and undeveloped ovary in L. vannamei. The microarray developed in this study can be a useful tools to uncover the molecular mechanisms in ovarian development in Penaeid shrimp species.

Free Research Field

水生生物繁殖生理