2017 Fiscal Year Annual Research Report
Comprehensive Omics analysis and functional characterization of the role of nervous system in the larval settlement of barnacle cyprids
| Project/Area Number |
16K18749
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| Research Institution | Akita Prefectural University |
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Principal Investigator |
黄 裕謙 秋田県立大学, 生物資源科学部, 特任助教 (80769547)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | Neuropeptides / Peptide hormones / Immunohistochemistry / Evolution / Larvae nervous system / SIFamide |
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| Outline of Annual Research Achievements |
109 neuropeptides and peptide hormone transcripts were recovered from the transcriptome of the acorn barnacle Megabalanus rosa. These include some known neuropeptides such as Allatostatins, Sulfakinin, FMRFamide family neuropeptides, pigment dispersal hormone and insulin like peptides. While the majority of these peptide hormones have been implicated in adult functions and behaviors, our result for the first time reveal their expression in barnacle cyprid larvae nervous system. By three color IHC experiments, the whole nervous system (anti-acetylated tubulin with red 2nd antibody), the location of SIFamide, Allatostatin and FMRFamide neuropeptides (anti-neuropeptide antibody with green 2nd antibodies) and larval anatomy (DAPI, blue) are simultaneously illustrated by three colors immunohistochemistry experiments. We further extend our sequence data to compare homologous neuropeptide sequences from other barnacle species and decapod crustaceans, through which we discover high degree of conservation. In particular, SIFamide exhibited similar spatial expression pattern in M.rosa and a distant clade the parasitic barnacle Sacculina yatsui. In M.rosa, SIFamide express not only in the major neural fiber, but also in distinct location of the larval body. Our results enable further study on the commonality of nervous system among different barnacle clades and therefore have major implication on the evolution of barnacle.
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Research Products
(8 results)
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[Journal Article] Identification, characterization, and larval biology of a rhizocephalan barnacle, Sacculina yatsui Boschma, 1936, from northwestern Japan (Cirripedia: Sacculinidae)2018
Author(s)
Kobayashi Momoko, Wong Yue-Him, Oguro-Okano Mieko, Dreyer Niklas, H; Jens T., Yoshida Ryuta and Okano Keiju
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Journal Title
Journal of Crustacean Biology
Volume: 38
Pages: 329-340
DOI
Peer Reviewed / Int'l Joint Research
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[Presentation] Identification of barnacle shell proteins by transcriptome and proteomic approaches2017
Author(s)
Yue Him Wong, Noriaki Ozaki, Wei-Pang Zhang, Jin Sun, Erina Yoshimura, Mieko Oguro-Okano, Yasuyuki Nogata, Hsiu-Chin Lin, Benny KK Chan, Pei-Yuan Qian, Keiju Okano
Organizer
The 14th International Symposium on Biomineralization (BIOMIN XIV) From Molecular and Nano-structural Analyses to Environmental Science
Int'l Joint Research / Invited
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