beta2-adrenergic receptor signaling regulates FOXO1 mRNA expression in skeletal muscle

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18784
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Animal production science
• Research Institution: Kagoshima University
• Principal Investigator: IJIRI Daichi 鹿児島大学, 農水産獣医学域農学系, 准教授 (50551090)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: 骨格筋 / タンパク質分解 / FOXO / microRNA

Outline of Final Research Achievements

The aims of this study were to investigate the role of beta2-adrenergic receptor (AR) signaling in regulation of FOXO1 mRNA expression in skeletal muscle. We showed that beta2-AR activation by using its selective agonist (clenbuterol) rapidly decreases FOXO1 mRNA expression both in chick myotubes and C2C12 myotubes. Further, we performed microRNA (miRNA) microarray analysis and identified that 103 miRNAs were significantly changed in C2C12 myotubes stimulated with 1 μM clenbuterol for 1 h. Two of the 103 miRNAs successfully decreased FoxO1 mRNA expression in C2C12 myotubes, respectively. Our results indicated that the beta2-AR signaling has a role to suppress FOXO1 mRNA expression via regulating miRNA expression.

Free Research Field

栄養生化学