The establishment for trypanocidal mechanism evaluation assay and application for the development for trypanocidal drugs

2019 Fiscal Year Annual Research Report

• Project/Area Number: 16K18793
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: 菅沼 啓輔 帯広畜産大学, 畜産学部, 助教 (60772184)
• Project Period (FY): 2016-04-01 – 2020-03-31
• Keywords: トリパノソーマ / 治療薬

Outline of Annual Research Achievements

研究代表者がこれまでに確立した代表的な動物トリパノソーマ病であるナガナ病（Trypanosoma congolense, T. brucei brucei), スーラ病（T. evansi）, 媾疫（こうえき）（T. equiperdum）及びヒトアフリカトリパノソーマ症（T. b. rhodesiense, T. b. gambiense）に対する薬剤スクリーニングによる新規抗トリパノソーマ化合物の探索とそれらの作用機序の解析のためのトランスクリプトームデータの解析を行っている。
今年度は昨年までに取得したT. equiperdumのトランスクリプトームデータの解析とその成果を国際学会での発表を行った。今後はさらなる薬剤探索とそれで見出された有用化合物の類縁体を用いたシード化合物の最適化、さらにはこれまでの研究で見出された新規抗トリパノソーマ化合物候補の動物モデルでの検証と作用機序解析を進めることでトリパノソーマ症治療薬の開発が期待される。

Research Products

• [Int'l Joint Research] モンゴル獣医学研究所(モンゴル)
  • Country Name: MONGOLIA
  • Counterpart Institution: モンゴル獣医学研究所
• [Int'l Joint Research] ノースウエスト大学(南アフリカ)
  • Country Name: SOUTH AFRICA
  • Counterpart Institution: ノースウエスト大学
• [Journal Article] Effect of vegetable oils on the experimental infection of mice with Trypanosoma congolense (2020)
  • Author(s): Kume Aiko、Suganuma Keisuke、Umemiya-Shirafuji Rika、Suzuki Hiroshi
  • Journal Title: Experimental Parasitology Volume: 210 Pages: 107845～107845
  • DOI: 10.1016/j.exppara.2020.107845
  • Peer Reviewed
• [Journal Article] Polyradiculoneuropathy in dourine-affected horses (2019)
  • Author(s): Mungun-Ochir Bayasgalan、Horiuchi Noriyuki、Altanchimeg Adilbish、Koyama Kenji、Suganuma Keisuke、Nyamdolgor Uranbileg、Watanabe Ken-ichi、Baatarjargal Purevdorj、Mizushima Daiki、Battur Banzragch、Yokoyama Naoaki、Battsetseg Badgar、Inoue Noboru、Kobayashi Yoshiyasu
  • Journal Title: Neuromuscular Disorders Volume: 29 Pages: 437～443
  • DOI: 10.1016/j.nmd.2019.03.005
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Short- and long-term effects of orally administered azithromycin on Trypanosoma brucei brucei-infected mice (2019)
  • Author(s): Molefe Nthatisi I.、Musinguzi Peter S.、Kondoh Daisuke、Watanabe Kenichi、Thekisoe Oriel M.M.、Xuan Xuenan、Inoue Noboru、Suganuma Keisuke
  • Journal Title: Experimental Parasitology Volume: 199 Pages: 40～46
  • DOI: 10.1016/j.exppara.2019.02.018
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Flavonoids isolated from the flowers of Pulsatilla flavescens and their anti-piroplasm activity. (2019)
  • Author(s): Ganchimeg D, Batbold B, Murata T, Davaapurev BO, Munkhjargal T, Tuvshintulga B, Suganuma K, Igarashi I, Buyankhishig B, Sasaki K, Batsuren D, Batkhuu J
  • Journal Title: Journal of natural medicines Volume: 73 Pages: 633-640
  • DOI: https://doi.org/10.1007/s11418-019-01294-8
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Acylated Lignans Isolated from Brachanthemum gobicum and Their Trypanocidal Activity (2019)
  • Author(s): Odonbayar Batsukh、Murata Toshihiro、Suganuma Keisuke、Ishikawa Yoshinobu、Buyankhishig Buyanmandakh、Batkhuu Javzan、Sasaki Kenroh
  • Journal Title: Journal of Natural Products Volume: 82 Pages: 774～784
  • DOI: 10.1021/acs.jnatprod.8b00670
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Dourine (Trypanosoma equiperdum infection in equidae) control campaign in Mongolia (2019)
  • Author(s): Keisuke Suganuma, Sandagdorj Narantsatsral, Batdorj Davaasuren, Daiki Mizushima, Badgar Batsetseg, Banzragch Battur, Noboru Inoue
  • Organizer: Conference on the cooperation and collaboration on prevention and control of animal disease
  • Int'l Joint Research / Invited
• [Presentation] Epidemiological studies on equine trypanosomosis in donkeys in Sudan (2019)
  • Author(s): Afraa Elata, Keisuke Suganuma, Ehab Mossaad, Rawan Satti, Xuenan Xuan, Noboru Inoue
  • Organizer: 第9回家畜感染症学会学術集会
• [Presentation] Draft Genome Sequence of Trypanosoma equiperdum IVM-t1 Strain (2019)
  • Author(s): Batdorj Davaasuren, Junya Yamagishi, Daiki Mizushima, Sandagdorj Narantsatsral, Davaajav Otgonsuren, Punsantsogvoo Myagmarsuren, Badgar Battsetseg, Banzragch Battur, Noboru Inoue, Keisuke Suganuma
  • Organizer: 4th international confernce on non-tsetse transmitted animal trypanosomes and trypanosomoses
  • Int'l Joint Research
• [Presentation] The screening of trypanocidal compounds extracted from Mongolian herbal plants against T. equiperdum Mongolian strains (2019)
  • Author(s): Keisuke Suganuma, Toshihiro Murata, Narantsatsral Sandagdorj, Orkhon Banzragchgarav, Batsukh Odonbayar, Davaasuren Batdorj, Javzan Batkhuu, Kenroh Sasaki, Banzragch Battur, Badgar Battsetseg, Noboru Inoue
  • Organizer: 4th international confernce on non-tsetse transmitted animal trypanosomes and trypanosomoses
  • Int'l Joint Research
• [Remarks] 帯広畜産大学原虫病研究センター
  • URL: https://www.obihiro.ac.jp/facility/protozoa/