Reconstitution of a novel recombinant Marek's disease virus as a vector vaccine against poultry red mites

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18798
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: Shiro Murata 北海道大学, 獣医学研究科, 助教 (10579163)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: ワクモ / 組換えワクチン / マレック病 / マレック病ウイルス / 感染性クローン / 組換えウイルス / 弱毒生ワクチン

Outline of Final Research Achievements

The poultry red mite (PRM), is one of the major hematophagous ectoparasites of poultry farming, and the decreased productivity due to blood-sucking by PRMs is a severe problem for the poultry industry. The use of acaricides often leads to the spread of acaricide-resistant PRMs. Therefore, alternative strategies are required to reduce economic losses in poultry farming. Here, we focus on preventive methods such as vaccination to control PRMs, and we have already identified several vaccine candidates. In order to prepare an easy-to-use anti-PRM vaccine, the recombinant Marek's disease (MD) virus (rMDV) expressing a vaccine antigen was reconstituted. Reconstituted rMDV showed no significant differences in the growth kinetics compared to the parental strain, and the vaccine antigen was expressed in the infected cells. Thus, the rMDV live vaccine expressing a vaccine antigen against PRMs may be applicable as a candidate dual vaccine that provides protection against both PRMs and MD.

Free Research Field

獣医伝染病学