Analysis of novel transcriptional suppressing mechanism in canine BRCA2

2018 Fiscal Year Final Research Report

• Project/Area Number: 16K18817
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: Kitasato University
• Principal Investigator: Yoshikawa Yasunaga 北里大学, 獣医学部, 講師 (00552043)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Keywords: 転写抑制因子 / BRCA2 / シス因子 / トランス因子

Outline of Final Research Achievements

In dogs and human, mutations and reduction expression level of BRCA2, one of tumor suppressor gene, are related to mammary tumor development. We had found a novel DNA region that suppresses BRCA2 promoter activity in preliminary study. In this study, we limited the region which include the suppressor activity to 50 bp length. A several proteins were interacted with this DNA sequence in vitro and one of them showed the suppressor effect on BRCA2 promoter activity.

Free Research Field

分子腫瘍学

Academic Significance and Societal Importance of the Research Achievements

BRCA2の変異や発現量の低下は腫瘍発症と関係している事がイヌならびにヒトで報告されている。すなわち、適切な発現量を維持することが重要である。本研究では、BRCA2の発現量を抑制するメカニズムを解明しようとするもので、新しい腫瘍発症の原因の解明に繋がることが期待される。