2017 Fiscal Year Final Research Report
Reconstitution of oocyte production system from pluripotent stem cells
| Project/Area Number |
16K18820
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Keywords | 卵巣 / 再構築 / マウスES細胞 / パターン形成 / 側板中胚葉 / 卵母細胞 |
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| Outline of Final Research Achievements |
I established a method to direct mouse embryonic stem cells (ESCs) to gonadal cells essential to reconstruct oogenesis. Optimal protocol was determined after preparation of several types of mouse reporter ESCs, which visualize the gonadal cell lineage differentiation. It is strongly expected that our induction method mimicked the process of embryonic gonadogenesis as following reasons. (i) mouse ESCs undergo differentiating steps (e.g. mesoderm and progenitor cells) in vitro in a similar developmental rate with gonadogenesis in vivo. (ii) Induction is led with molecules working for gonadogenesis. (iii) Cells in embryoid body differentiate in a similar spatial pattern and show almost same reorter activity with that in mouse embryo. These results must be an important help to the oocyte induction from a variety of animals.
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| Free Research Field |
発生生物学
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