Exploitation of proton pumping ATPase inhibitors in oral cavity pathogenic bacteria

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18877
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biological pharmacy
• Research Institution: Iwate Medical University
• Principal Investigator: Sekiya Mizuki 岩手医科大学, 薬学部, 助教 (70509033)
• Research Collaborator: 岩本 昌子 長浜バイオ大学, バイオサイエンス学部, 准教授 ; 佐々木 実 岩手医科大学, 歯学部, 教授 ; 阪本 泰光 岩手医科大学, 薬学部, 准教授
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: プロトン輸送ATPase / F-ATPase / A-ATPase / ポリフェノール / Curcumin / 虫歯 / 歯周病

Outline of Final Research Achievements

In this study, we investigated the roles of proton pump ATPases in the principal causative agent of human dental deseases, S. mutans and P. gingivalis.
We suggested that F-ATPase is an essential role in acid tolerance in S. mutans, and proton gradient and A-ATPase are important for the growth of P. gingivalis. We also found various inhibitors of bacterial proton pump ATPase and suggested their inhibitory mechanism and binding sites.
Furthermore, we analyze the function of lysosomal proton pump V-ATPase in mammalian osteoclast, which associates with bone resorption in periodontal diseases. We found a novel role for the V-ATPase in secretory lysosome trafficking and an unexpected mechanistic link with Rab GTPases.

Free Research Field

機能生化学