Molecular cloning and characterization of a Perilla frutescens enzyme that catalyzes the essential oil biosynthesis

2017 Fiscal Year Final Research Report

• Project/Area Number: 16K18902
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Natural medicines
• Research Institution: Kinjo Gakuin University
• Principal Investigator: Fujiwara Yumi 金城学院大学, 薬学部, 助教 (90756511)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Keywords: シソ属植物 / 精油成分 / シトクロムP450 / ペリルアルデヒド / クローニング / 機能的発現 / 酵素反応

Outline of Final Research Achievements

Perilla is the source of several oil types. A library of genetically pure lines of perilla has been established and many studies has been conducted showing that the syntheses of oil compounds are genetically controlled. With this library of pure strains of perilla, it is possible to compare expression levels of specific genes in different oil types and identify sequences relevant to the syntheses of oil compounds specific to the oil type. Herein described as the cloning and characterization of a cytochrome P450 enzyme mediating the synthesis of perillaldehyde. The recombinant protein catalyzed the hydroxylation and oxidation of limonene to perillyl alcohol and perillaldehyde.

Free Research Field

生薬学